ypbE [2019-08-26 09:16:06]

peptidoglycan-binding protein, likely involved in sidewall synthesis during cell growth

locus: BSU_23000

pI: 5.43

mw: 27.14 kDa

protein length: 240 aa

gene length: 723 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,405,620 → 2,406,342

The protein

[SW|Domains]

• contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] (aa 189-235) [Pubmed|18430080]

Effectors of protein activity

• binding to peptidoglycan is stimulated by YpbE multimerisation, induced by hexameric [protein|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|GpsB] [pubmed|30651563]

[SW|Localization]

• cell membrane [pubmed|30651563]

Biological materials

Mutant

• MGNA-A395 (ypbE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/395 NBRP B. subtilis, Japan]
• BKE23000 (Δ[gene|CF81E3A0CD4A65B29583E7F7D85C9B59D8C7791C|ypbE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCCGCCAATGCGGCGTTTT, downstream forward: _UP4_TAGGGTATTATTTTTATTTT
• BKK23000 (Δ[gene|CF81E3A0CD4A65B29583E7F7D85C9B59D8C7791C|ypbE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCCGCCAATGCGGCGTTTT, downstream forward: _UP4_TAGGGTATTATTTTTATTTT

References

Reviews

• 18430080

Research papers

• 30651563